Immunohistochemical Evidence of Melatonin Protection on Lung Tissue During Chemotherapy

The present study investigated the possible protective effects of melatonin on Bleomycin, Cisplatin and etoposide (BEP) chemotherapy regimens using immunohistochemistry. Forty male Wistar rats were divided into four groups ten as; group 1 as untreated control; 2 BEP which received three cycles 21 days’ regimen each 0.5¥ dose levels ofBEP (bleomycin 0.75 mg/kg, 7.5 mg/kg cisplatin 1.5 mg/kg). Rats in 3 (MEL group) 10 mg/kg/day once daily. Group 4 (30 min before injections) 2. Proliferating cell nuclear antigen (PCNA) staining was used to detect proliferation caspase-3, caspase-9 Caspase-8 detected investigate apoptosis. PCNA immunostaining alveolar epithelium, macrophages bronchus weak moderate group. However, diffuse strong caspase immunoreactions for 8- bronchioles vascular endothelium, luminal immunoreactivities MEL Mel + close control one. surface are significantly reduced compared one ((P<0.05). groups, it value (P>0.05). It can be concluded that affects negatively lung tissue inhibits injuries during chemotherapy.